Cell biological techniques
Institut |
Technology; |
Infrastructure |
Contact Person |
BNITM
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Functional genetic screens in human haploid cells | This email address is being protected from spambots. You need JavaScript enabled to view it. | |
Model system for host cell-pathogen interactions (dictyostelium)
- phagosome maturation- Life Cell imaging, - molecular biology |
Olympus Imaging Station (BSL2) | ||
hormone manipulation, Immunohistology (PFA liver), Isolation of protozoa from liver tissue, Magnet Particle labeling, Quantum dot labeling, FACS sorting, intracellular FACS of human NKT cells |
Gonadectomy
Substitution AK Keyence Biorevo Overview |
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Gene Exchange Mutagenesis in Leishmania spp. | S2/S3* Genetic engineering plant | This email address is being protected from spambots. You need JavaScript enabled to view it. | |
Leishmania complementation genetics (knock-in and episomal) | S2/S3* Genetic engineering plant | This email address is being protected from spambots. You need JavaScript enabled to view it. | |
Drosophila and mosquito cell lines |
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FZB |
Bronchoalveolar lavage (BAL) Elutriation
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Flow cytometric characterization of pathogens and isolated pathogen-containing compartments |
FACS Canto II, BSL3 based flow cytometer (96 well format) |
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Trafficking of intracellular bacterial pathogens in primary cells, Mtb reporter strains, life cell imaging, bead phagosome model, ex vivo precision cut lung slices, biofilm assays |
Nikon Biostation (BSL3), Nikon Life Cell Mikroskop (BSL2), Vibratom
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Epithelial cell barrier measurement, ex vivo intestinal crypt organoids |
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LIV |
Production of recombinant influenza viruses using reverse genetics, intracellular nuclear transport | BSL-2, BSL3 and BSL3+; cell lines with deleted nuclear transport genes | This email address is being protected from spambots. You need JavaScript enabled to view it. |